abstract：:The aim of the present study was to measure free thiols content, Na(+)/K(+)-ATPase and Ca(2+)-ATPase activities in human spermatozoa of asthenozoospermic patients and normospermic donors, and evaluate any influence on kinetic sperm features, as well as correlation with peroxynitrite. In fact, membrane integrity and it...

journal_title：Reproductive biomedicine online

authors： Vignini A,Buldreghini E,Nanetti L,Amoroso S,Boscaro M,Ricciardo-Lamonica G,Mazzanti L,Balercia G

更新日期：2009-01-01 00:00:00

abstract：:Oocyte cryopreservation has long been the focus of unsuccessful efforts to perfect its clinical application. Lately, vitrification has been shown to provide high degrees of success by different research groups. This review attempts to address the efficiency of oocyte vitrification in clinical practice through an analy...

journal_title：Reproductive biomedicine online

authors： Cobo A,Vajta G,Remohí J

更新日期：2009-01-01 00:00:00

abstract：:This study describes and compares the possible effects of vitrification on the ultrastructural morphology of 20 human mature oocytes vitrified using two different supports, cryoleaf (n = 10) and cryoloop (n = 10). Fresh human mature oocytes (n = 15) were used as controls. Fresh and vitrified-warmed oocytes appeared ro...

journal_title：Reproductive biomedicine online

authors： Nottola SA,Coticchio G,Sciajno R,Gambardella A,Maione M,Scaravelli G,Bianchi S,Macchiarelli G,Borini A

更新日期：2009-01-01 00:00:00

abstract：:Countries that oppose embryo destruction for research purposes can forbid the production of human embryonic stem cell lines on their territory, but these cell lines are nevertheless produced abroad. Therefore, these countries also need to determine if their position on embryo destruction allows them to import and use ...

journal_title：Reproductive biomedicine online

authors： Mertes H,Pennings G

更新日期：2009-01-01 00:00:00

abstract：:The predictive value of pre-ovulatory hormones for achievement of pregnancy has been extensively evaluated in assisted reproductive technologies. Currently, no high-quality evidence exists to support or deny an association between oestradiol levels on the day of human chorionic gonadotrophin (HCG) administration and p...

journal_title：Reproductive biomedicine online

authors： Kolibianakis EM,Venetis CA,Tarlatzis BC

更新日期：2009-01-01 00:00:00

abstract：:Human amniotic fluid cells have been used as a diagnostic tool for the prenatal diagnosis of fetal genetic anomalies for more than 50 years. Evidence provided in the last 5 years, however, suggests that they can also harbour a therapeutic potential for human diseases, as different populations of fetal-derived stem cel...

journal_title：Reproductive biomedicine online

authors： Cananzi M,Atala A,De Coppi P

更新日期：2009-01-01 00:00:00

abstract：:Total fertilization failures (TFF) are rare events of IVF by intracytoplasmic sperm injection (ICSI). When male factor is excluded, the lack of identifiable aetiological criteria raises the question of the reliable clinical management. The goal of this study was to identify molecular abnormalities in metaphase II (MII...

journal_title：Reproductive biomedicine online

authors： Gasca S,Reyftmann L,Pellestor F,Rème T,Assou S,Anahory T,Dechaud H,Klein B,De Vos J,Hamamah S

更新日期：2008-12-01 00:00:00

abstract：:This randomized, controlled trial verifies whether patients with recurrent failed implantation benefit from preimplantation genetic diagnosis for aneuploidy, as compared with conventional assisted reproduction treatment procedures. Two hundred patients with recurrent failed implantation were randomized into two groups...

journal_title：Reproductive biomedicine online

authors： Blockeel C,Schutyser V,De Vos A,Verpoest W,De Vos M,Staessen C,Haentjens P,Van der Elst J,Devroey P

更新日期：2008-12-01 00:00:00

abstract：:Failed fertilization after intracytoplasmic sperm injection (ICSI) can occur due to an oocyte activation defect. In these cases assisted oocyte activation (AOA) may help but efficiency is still unknown. Prior to AOA, the mouse oocyte activation test (MOAT) can be carried out by injecting human spermatozoa into mouse o...

journal_title：Reproductive biomedicine online

authors： Heindryckx B,De Gheselle S,Gerris J,Dhont M,De Sutter P

更新日期：2008-11-01 00:00:00

abstract：:Poor ovarian response in IVF cycles is associated with poor pregnancy rates. Expected poor responders may represent the worst prognostic group. Data were used from 222 patients starting the first of three IVF treatment cycles. The predictability of ongoing pregnancy after three cycles was analysed using survival analy...

journal_title：Reproductive biomedicine online

authors： Hendriks DJ,te Velde ER,Looman CW,Bancsi LF,Broekmans FJ

更新日期：2008-11-01 00:00:00

abstract：:Non-invasive methods of assessing embryo quality are critical for pregnancy success following IVF or intracytoplasmic sperm injection (ICSI). The addition of new non-invasive morphological and biochemical analyses may further improve pregnancy success, allowing the transfer of a single embryo, thereby reducing the ris...

journal_title：Reproductive biomedicine online

authors： Warner CM,Lampton PW,Newmark JA,Cohen J

更新日期：2008-10-01 00:00:00

abstract：:The aim of this study was to compare the efficacy of two embryo transfer catheters: Wallace and Rocket Embryon in an IVF programme of a tertiary referral university centre. A total of 308 patients undergoing embryo transfer were prospectively randomized to either a transfer with the Wallace catheter or a transfer with...

journal_title：Reproductive biomedicine online

authors： El-Shawarby SA,Ravhon A,Skull J,Ellenbogen A,Trew G,Lavery S

更新日期：2008-10-01 00:00:00

abstract：:Ovarian hyperstimulation syndrome (OHSS) is a rare but potentially fatal complication of IVF treatment. The risk of OHSS increases with increasing numbers of follicles aspirated and oocytes retrieved, but there is little evidence to support whether threshold values of either can be used to correctly predict OHSS. Sinc...

journal_title：Reproductive biomedicine online

authors： Verwoerd GR,Mathews T,Brinsden PR

更新日期：2008-09-01 00:00:00

abstract：:More than 17,000 intrauterine insemination (lUI) cycles were analysed retrospectively with respect to outcome according to differing aetiologies of infertility. The quantity and motility of spermatozoa in the final preparation used for insemination had a positive effect on the outcome, as classically observed in the p...

journal_title：Reproductive biomedicine online

authors： Belloc S,Cohen-Bacrie P,Benkhalifa M,Cohen-Bacrie M,De Mouzon J,Hazout A,Ménézo Y

更新日期：2008-09-01 00:00:00

abstract：:Progesterone has been implicated in the process of spermatogenesis. This study aimed to investigate the association of progesterone receptor (PR) expression with spermatogenesis in the testis of infertile men. PR mRNA and protein were assessed by in-situ hybridization and immunohistochemistry in testicular biopsies ob...

journal_title：Reproductive biomedicine online

authors： Abid S,Gokral J,Maitra A,Meherji P,Kadam S,Pires E,Modi D

更新日期：2008-08-01 00:00:00

abstract：:The aim of this study was to determine the correlation between three-dimensional power Doppler sonography (3D-PDS) of the (sub)endometrium and concentrations of angiogenic cytokines in patients attending an IVF programme. A total of 42 patients was included in a prospective, non-randomized clinical study. 3D-PDS of th...

journal_title：Reproductive biomedicine online

authors： Siemer J,Binder H,Willeke C,Dorn C,Reinsberg J,Fimmers R,van der Ven H,Schild RL

更新日期：2008-08-01 00:00:00

abstract：:An effective, consistent and xeno-free cryopreservation technique is crucial for any human embryonic stem cell (hESC) laboratory with future perspectives for clinical application. This study presents a new slow freezing-rapid thawing method in serum-free conditions that allows the cryopreservation of a large number of...

journal_title：Reproductive biomedicine online

authors： Valbuena D,Sánchez-Luengo S,Galán A,Sánchez E,Gómez E,Poo ME,Ruiz V,Genbacev O,Krtolica A,Pellicer A,Moreno R,Simón C

更新日期：2008-07-01 00:00:00

abstract：:Osteopontin and its receptor alpha(v)beta(3) integrin have recently been proposed as a major complex to promote embryo attachment, and thus they would be useful as markers of endometrial receptivity. In the current study alpha(v)beta(3) integrin and osteopontin expression and co-expression in in-phase and out-of-phase...

journal_title：Reproductive biomedicine online

authors： Casals G,Ordi J,Creus M,Fábregues F,Casamitjana R,Quinto L,Campo E,Balasch J

更新日期：2008-06-01 00:00:00

abstract：:Serum anti-Müllerian hormone (AMH) concentrations decline with increasing age and constitute a sensitive marker for ovarian ageing. In addition, basal serum AMH concentrations predict ovarian response during IVF cycles. Concomitantly, oocyte quantity and embryo quality decrease with advancing age. Hence, it was postul...

journal_title：Reproductive biomedicine online

authors： Lie Fong S,Baart EB,Martini E,Schipper I,Visser JA,Themmen AP,de Jong FH,Fauser BJ,Laven JS

更新日期：2008-05-01 00:00:00

abstract：:Cytogenetic investigations are performed in couples asking for IVF or intracytoplasmic sperm injection (ICSI) treatment. These serve a diagnostic purpose because male or female infertility might have a chromosomal origin. Chromosomal aberrations found in these patients include numerical abnormalities, such as Klinefel...

journal_title：Reproductive biomedicine online

authors： Chantot-Bastaraud S,Ravel C,Siffroi JP

更新日期：2008-04-01 00:00:00

abstract：:The aim of this study was to determine if assisted hatching (AH) could improve the rates of pregnancy and implantation for both fresh and frozen-thawed embryo transfer cycles. A total of 760 fresh embryo transfer cycles and 200 frozen-thawed embryo transfer cycles were randomly assigned to either the treatment group (...

journal_title：Reproductive biomedicine online

authors： Ge HS,Zhou W,Zhang W,Lin JJ

更新日期：2008-04-01 00:00:00

abstract：:A preliminary study was conducted on health of children conceived after preimplantation genetic screening and diagnosis (PGD). Forty-nine children were assessed with 66 matched naturally conceived (NC) controls. Primary outcome was neurodevelopmental screening, and secondary outcomes were evidence of other health prob...

journal_title：Reproductive biomedicine online

authors： Banerjee I,Shevlin M,Taranissi M,Thornhill A,Abdalla H,Ozturk O,Barnes J,Sutcliffe A

更新日期：2008-03-01 00:00:00

abstract：:The introduction to this review discusses briefly why immunology, perceived as difficult by assisted reproduction technology clinicians, need nevertheless be envisaged as a central actor in the reproduction process, and how the maternal immune system, initially perceived as a threat to the fetoplacental unit, is in fa...

journal_title：Reproductive biomedicine online

authors： Chaouat G

更新日期：2008-02-01 00:00:00

abstract：:A retrospective study recently showed that oocytes presenting with a high birefringence of the inner zona layer were more often associated with conception cycles. To further investigate these findings, a prospective study was conducted between September 2005 and September 2006 including intracytoplasmic sperm injectio...

journal_title：Reproductive biomedicine online

authors： Montag M,Schimming T,Köster M,Zhou C,Dorn C,Rösing B,van der Ven H,Ven der Ven K

更新日期：2008-02-01 00:00:00

abstract：:Tubal pathology is a common cause of subfertility. Identifying risk factors for tubal pathology in the medical history is important to distinguish between those couples who benefit from early tubal patency tests and those in whom presence of tubal pathology is less likely and delaying tubal tests is justified. This st...

journal_title：Reproductive biomedicine online

authors： Verhoeve HR,Steures P,Flierman PA,van der Veen F,Mol BW

更新日期：2008-02-01 00:00:00

abstract：:The aim of this descriptive study was to evaluate the attitudes and knowledge of IVF staff towards oocyte donation (OD) programme. A total of 163 individuals (19% men and 81% women) were requested to fill out a two-part questionnaire. Part 1 of the questionnaire contained general demographic information: 62% of the pa...

journal_title：Reproductive biomedicine online

authors： Khalili MA,Isikoglu M,Tabibnejad N,Ahmadi M,Abed F,Parsanejad ME,Ghasemi M,el Jamal H

更新日期：2008-01-01 00:00:00

abstract：:Regenerative medical therapy has been expected to compensate for the therapeutic disadvantages of reconstructive surgery and organ transplantation, as well as create a new therapeutic strategy. The objective of regenerative medical therapy is to induce the regeneration and repair of defective tissues based on the natu...

journal_title：Reproductive biomedicine online

authors： Tabata Y

更新日期：2008-01-01 00:00:00

abstract：:The Preimplantation Genetic Diagnosis International Society (PGDIS) was organized in October 2002, with the purpose of encouraging and co-ordinating research, education and training in this multidisciplinary field, requiring a close collaboration of obstetricians, fertility specialists, embryologists and human genetic...

journal_title：Reproductive biomedicine online

authors： Preimplantation Genetic Diagnosis International Society (PGDIS).

更新日期：2008-01-01 00:00:00

abstract：:This paper examines the regulatory framework currently governing the creation of animal-human hybrids and chimera embryos in stem cell research, and some of the ethical implications of such research. It discusses the findings of a recent government select committee that considered the topic. It considers the debate ar...

journal_title：Reproductive biomedicine online

authors： Bahadur G,Iqbal M,Malik S,Sanyal A,Wafa R,Noble R

更新日期：2008-01-01 00:00:00

abstract：:It has been suggested that during the past five decades human sperm counts have declined and the incidence of testicular cancer, hypospadias and cryptorchidism has increased. Furthermore, geographical differences, with respect to these markers of male reproductive function, have been reported. According to a recent hy...

journal_title：Reproductive biomedicine online

authors： Giwercman A,Rylander L,Lundberg Giwercman Y

更新日期：2007-12-01 00:00:00

abstract：:The Catholic Church has established a large number of universities worldwide. A dozen have schools of medicine and the impact of church doctrine on reproductive medicine appears widely disparate between some medical schools at Catholic universities in Europe and the rest of the world. The pressure of the Vatican on th...

journal_title：Reproductive biomedicine online

authors： Brosens I

更新日期：2007-12-01 00:00:00

abstract：:A mounting interest in natural cycle IVF has challenged the medical community to better understand the mechanisms controlling the follicular phase and ovulation in particular, in an effort to optimize this procedure and its outcome. For practical reasons, the advancement of the follicular phase in the menstrual cycle ...

journal_title：Reproductive biomedicine online

authors： de Ziegler D,Fraisse T,de Candolle G,Vulliemoz N,Bellavia M,Colamaria S

更新日期：2007-11-01 00:00:00

abstract：:Anticancer effects of statins were studied using karyotypically normal human embryonic stem cells (hESC) (HES3), karyotypically abnormal hESC (BG0IV), embryonal carcinoma (NTERA-2), ovarian (TOV-112D) and colorectal cancer (HT-29) cells. The cells were treated with simvastatin, pravastatin, mevastatin and lovastatin i...

journal_title：Reproductive biomedicine online

authors： Gauthaman K,Richards M,Wong J,Bongso A

更新日期：2007-11-01 00:00:00

abstract：:The techniques currently used to treat infertility cases are quite limited in their capabilities, due to an incomplete understanding of the molecular activities of germ cells. Fortunately, several technologies are presently being researched that should aid the understanding of the various molecular causes of germ cell...

journal_title：Reproductive biomedicine online

authors： Varghese AC,Goldberg E,Bhattacharyya AK,Agarwal A

更新日期：2007-10-01 00:00:00

abstract：:Cervical mucus may cover the embryo transfer catheter during passage of the cervical canal, interfering with the correct placement of the embryo(s) into the uterine cavity. The effect of removal of cervical mucus prior to embryo transfer in IVF/ intracytoplasmic sperm injection (ICSI) on live birth rate was studied. T...

journal_title：Reproductive biomedicine online

authors： Visschers BA,Bots RS,Peeters MF,Mol BW,van Dessel HJ

更新日期：2007-09-01 00:00:00

abstract：:The aim of this study was to evaluate the impact of gonadotrophin therapy in combination with intracytoplasmic sperm injection (ICSI) in men with hypogonadotrophic hypogonadism (HH). Twenty-five azoospermic men were diagnosed with HH due to low FSH, LH and total testosterone concentrations. These patients were treated...

journal_title：Reproductive biomedicine online

authors： Bakircioglu ME,Erden HF,Ciray HN,Bayazit N,Bahçeci M

更新日期：2007-08-01 00:00:00

abstract：:In this prospective randomized study, women with idiopathic karyotypically normal premature ovarian failure (POF) were treated with gonadotrophin-releasing hormone (GnRH) agonist and gonadotrophins with and without the addition of corticosteroids in an attempt to restore ovarian function. The study comprised 58 women ...

journal_title：Reproductive biomedicine online

authors： Badawy A,Goda H,Ragab A

更新日期：2007-08-01 00:00:00

abstract：:The mammalian oocyte is especially sensitive to cryopreservation. Because of its size and physiology, it can easily undergo cell death or sub-lethal damage as a consequence of intracellular ice formation, increase in the concentration of solutes and other undesired effects during the conversion of extracellular water ...

journal_title：Reproductive biomedicine online

authors： Coticchio G,Bonu MA,Sciajno R,Sereni E,Bianchi V,Borini A

更新日期：2007-07-01 00:00:00

abstract：:The objective of this study was to investigate whether a change in assisted hatching technique from partial opening to total removal of the zona pellucida improved the outcome of vitrified blastocyst transfer. This was a preliminary observational study conducted from November 2003 to April 2006. Partial opening using ...

journal_title：Reproductive biomedicine online

authors： Hiraoka K,Fuchiwaki M,Hiraoka K,Horiuchi T,Murakami T,Kinutani M,Kinutani K

更新日期：2007-07-01 00:00:00

abstract：:Although mammalian ovaries contain hundreds of thousands of pre-antral follicles, fewer than 1% of these reach maturity and ovulation. Obtaining immature eggs from the pre-antral follicles of ovarian tissue could increase the possibility of preserving fertility in women undergoing anti-cancer treatment, and in women w...

journal_title：Reproductive biomedicine online

authors： Kagawa N,Kuwayama M,Nakata K,Vajta G,Silber S,Manabe N,Kato O

更新日期：2007-06-01 00:00:00